Fluorescence:Article Title: Generating GABAergic neurons in brains
Article Snippet: Antibodies The following primary antibodies were used: polyclonal anti-green fluorescent protein (GFP, chicken, 1:2000, Abcam, AB13970), polyclonal anti-glial fibrillary acidic protein (GFAP, rabbit, 1:1000, Dako, Z0334), monoclonal anti-CNPase (mouse, 1:800, Abcam, AB6319), polyclonal anti-vesicular glutamate transporter 1 (vGluT1, rabbit, 1:1000, Synaptic Systems), monoclonal anti GAD67 (mouse, 1:1000, Millipore, MAB5406), monoclonal anti GAD65 (GAD-6, mouse, 1:1000, Developmental Studies Hybridoma Bank, Iowa City), monoclonal anti-NG2 (mouse, 1:600, Abcam, AB50009), polyclonal anti-Iba1 (rabbit, 1:1000, Wako, 019-19741), polyclonal anti-NeuN (rabbit, 1:1000, Millipore, ABN78), monoclonal anti-βIII tubulin (Tuj1, mouse, 1:1000, COVANCE, MMS-435P), polyclonal anti-γ-aminobutyric acid (GABA, rabbit, 1:2000, Sigma, A2052), polyclonal anti-Red Fluorescent Protein (RFP, rat, 1:1500, antibody-online, ABIN334653; and rabbit, 1:1000, Rockland, 600-401-379), polyclonal anti-T-box brain 1 (Tbr1, 1:800, rabbit, Abcam, AB31940), monoclonal anti-Ctip2 (Rat, 1:1000, Abcam, AB18465), monoclonal anti-NeuroD1 (mouse, 1:1000, Abcam, AB60704), polyclonal anti-Dlx2 (rabbit, 1:600, Abcam, AB30339), polyclonal anti-Doublecortin (DCX, rabbit, 1:1000, Abcam, AB18723), monoclonal anti-Parvalbumin (PV, mouse, 1:1000, sigma, P3088), polyclonal anti-Calretinin (CR, goat, 1:1500, Millipore, AB1550), monoclonal anti-Somatostatin (SST, rat, 1:300, Millipore, MAB354), polyclonal anti-Cholecystokinin (Catalog Nos.
Article Title: Directing Astroglia from the Cerebral Cortex into Subtype Specific Functional Neurons
Article Snippet: The following primary antibodies were used: anti-GFP (GFP, chicken, 1∶1000, Aves Labs, GFP-1020 ), polyclonal anti-Glial Fibrillary Acidic Protein (GFAP, rabbit, 1∶4000, DakoCytomation, Z0334 ), polyclonal anti-Red Fluorescent Protein (RFP, rabbit, 1∶500, Chemicon, AB3216 ), polyclonal anti-RFP (rabbit, Rockland, 1∶2000, 600-401-379 ), polyclonal anti-vesicular glutamate transporter 1 (vGluT11, rabbit, 1∶1000, Synaptic Systems, 135302 ), monoclonal anti-Microtubule Associated Protein 2 (MAP2, mouse IgG1, 1∶200, Sigma-Aldrich, M4403 ), monoclonal anti-synapsin 1 (mouse IgG2, 1∶2000, Synaptic Systems, 106001 ), polyclonal anti-vGaT (guinea pig, 1∶200, Synaptic Systems, 131004 ), polyclonal anti-Tbr1 (rabbit, 1∶1000, Millipore, AB9616), polyclonal anti-Tbr2 (rabbit, 1∶500, Millipore, AB9618), monoclonal anti-βIII tubulin (mouse IgG2b, 1∶500, Sigma, T8660), polyclonal anti-GAD1(GAD67) (rabbit, 1∶500, Synaptic Systems, 198013), monoclonal anti-calretinin (mouse IgG1, 1∶200, Millipore, MAB1568), and monoclonal anti-CaM kinase IIα (mouse IgG1, 1∶200, Abcam, ab2725).
Derivative Assay:Article Title: Generating GABAergic neurons in brains
Article Snippet: Antibodies The following primary antibodies were used: polyclonal anti-green fluorescent protein (GFP, chicken, 1:2000, Abcam, AB13970), polyclonal anti-glial fibrillary acidic protein (GFAP, rabbit, 1:1000, Dako, Z0334), monoclonal anti-CNPase (mouse, 1:800, Abcam, AB6319), polyclonal anti-vesicular glutamate transporter 1 (vGluT1, rabbit, 1:1000, Synaptic Systems), monoclonal anti GAD67 (mouse, 1:1000, Millipore, MAB5406), monoclonal anti GAD65 (GAD-6, mouse, 1:1000, Developmental Studies Hybridoma Bank, Iowa City), monoclonal anti-NG2 (mouse, 1:600, Abcam, AB50009), polyclonal anti-Iba1 (rabbit, 1:1000, Wako, 019-19741), polyclonal anti-NeuN (rabbit, 1:1000, Millipore, ABN78), monoclonal anti-βIII tubulin (Tuj1, mouse, 1:1000, COVANCE, MMS-435P), polyclonal anti-γ-aminobutyric acid (GABA, rabbit, 1:2000, Sigma, A2052), polyclonal anti-Red Fluorescent Protein (RFP, rat, 1:1500, antibody-online, ABIN334653; and rabbit, 1:1000, Rockland, 600-401-379), polyclonal anti-T-box brain 1 (Tbr1, 1:800, rabbit, Abcam, AB31940), monoclonal anti-Ctip2 (Rat, 1:1000, Abcam, AB18465), monoclonal anti-NeuroD1 (mouse, 1:1000, Abcam, AB60704), polyclonal anti-Dlx2 (rabbit, 1:600, Abcam, AB30339), polyclonal anti-Doublecortin (DCX, rabbit, 1:1000, Abcam, AB18723), monoclonal anti-Parvalbumin (PV, mouse, 1:1000, sigma, P3088), polyclonal anti-Calretinin (CR, goat, 1:1500, Millipore, AB1550), monoclonal anti-Somatostatin (SST, rat, 1:300, Millipore, MAB354), polyclonal anti-Cholecystokinin (Catalog Nos.
Article Title: Directing Astroglia from the Cerebral Cortex into Subtype Specific Functional Neurons
Article Snippet: The following primary antibodies were used: anti-GFP (GFP, chicken, 1∶1000, Aves Labs, GFP-1020 ), polyclonal anti-Glial Fibrillary Acidic Protein (GFAP, rabbit, 1∶4000, DakoCytomation, Z0334 ), polyclonal anti-Red Fluorescent Protein (RFP, rabbit, 1∶500, Chemicon, AB3216 ), polyclonal anti-RFP (rabbit, Rockland, 1∶2000, 600-401-379 ), polyclonal anti-vesicular glutamate transporter 1 (vGluT11, rabbit, 1∶1000, Synaptic Systems, 135302 ), monoclonal anti-Microtubule Associated Protein 2 (MAP2, mouse IgG1, 1∶200, Sigma-Aldrich, M4403 ), monoclonal anti-synapsin 1 (mouse IgG2, 1∶2000, Synaptic Systems, 106001 ), polyclonal anti-vGaT (guinea pig, 1∶200, Synaptic Systems, 131004 ), polyclonal anti-Tbr1 (rabbit, 1∶1000, Millipore, AB9616), polyclonal anti-Tbr2 (rabbit, 1∶500, Millipore, AB9618), monoclonal anti-βIII tubulin (mouse IgG2b, 1∶500, Sigma, T8660), polyclonal anti-GAD1(GAD67) (rabbit, 1∶500, Synaptic Systems, 198013), monoclonal anti-calretinin (mouse IgG1, 1∶200, Millipore, MAB1568), and monoclonal anti-CaM kinase IIα (mouse IgG1, 1∶200, Abcam, ab2725).
Transduction:Article Title: Generating GABAergic neurons in brains
Article Snippet: Antibodies The following primary antibodies were used: polyclonal anti-green fluorescent protein (GFP, chicken, 1:2000, Abcam, AB13970), polyclonal anti-glial fibrillary acidic protein (GFAP, rabbit, 1:1000, Dako, Z0334), monoclonal anti-CNPase (mouse, 1:800, Abcam, AB6319), polyclonal anti-vesicular glutamate transporter 1 (vGluT1, rabbit, 1:1000, Synaptic Systems), monoclonal anti GAD67 (mouse, 1:1000, Millipore, MAB5406), monoclonal anti GAD65 (GAD-6, mouse, 1:1000, Developmental Studies Hybridoma Bank, Iowa City), monoclonal anti-NG2 (mouse, 1:600, Abcam, AB50009), polyclonal anti-Iba1 (rabbit, 1:1000, Wako, 019-19741), polyclonal anti-NeuN (rabbit, 1:1000, Millipore, ABN78), monoclonal anti-βIII tubulin (Tuj1, mouse, 1:1000, COVANCE, MMS-435P), polyclonal anti-γ-aminobutyric acid (GABA, rabbit, 1:2000, Sigma, A2052), polyclonal anti-Red Fluorescent Protein (RFP, rat, 1:1500, antibody-online, ABIN334653; and rabbit, 1:1000, Rockland, 600-401-379), polyclonal anti-T-box brain 1 (Tbr1, 1:800, rabbit, Abcam, AB31940), monoclonal anti-Ctip2 (Rat, 1:1000, Abcam, AB18465), monoclonal anti-NeuroD1 (mouse, 1:1000, Abcam, AB60704), polyclonal anti-Dlx2 (rabbit, 1:600, Abcam, AB30339), polyclonal anti-Doublecortin (DCX, rabbit, 1:1000, Abcam, AB18723), monoclonal anti-Parvalbumin (PV, mouse, 1:1000, sigma, P3088), polyclonal anti-Calretinin (CR, goat, 1:1500, Millipore, AB1550), monoclonal anti-Somatostatin (SST, rat, 1:300, Millipore, MAB354), polyclonal anti-Cholecystokinin (Catalog Nos.
Article Title: Directing Astroglia from the Cerebral Cortex into Subtype Specific Functional Neurons
Article Snippet: The following primary antibodies were used: anti-GFP (GFP, chicken, 1∶1000, Aves Labs, GFP-1020 ), polyclonal anti-Glial Fibrillary Acidic Protein (GFAP, rabbit, 1∶4000, DakoCytomation, Z0334 ), polyclonal anti-Red Fluorescent Protein (RFP, rabbit, 1∶500, Chemicon, AB3216 ), polyclonal anti-RFP (rabbit, Rockland, 1∶2000, 600-401-379 ), polyclonal anti-vesicular glutamate transporter 1 (vGluT11, rabbit, 1∶1000, Synaptic Systems, 135302 ), monoclonal anti-Microtubule Associated Protein 2 (MAP2, mouse IgG1, 1∶200, Sigma-Aldrich, M4403 ), monoclonal anti-synapsin 1 (mouse IgG2, 1∶2000, Synaptic Systems, 106001 ), polyclonal anti-vGaT (guinea pig, 1∶200, Synaptic Systems, 131004 ), polyclonal anti-Tbr1 (rabbit, 1∶1000, Millipore, AB9616), polyclonal anti-Tbr2 (rabbit, 1∶500, Millipore, AB9618), monoclonal anti-βIII tubulin (mouse IgG2b, 1∶500, Sigma, T8660), polyclonal anti-GAD1(GAD67) (rabbit, 1∶500, Synaptic Systems, 198013), monoclonal anti-calretinin (mouse IgG1, 1∶200, Millipore, MAB1568), and monoclonal anti-CaM kinase IIα (mouse IgG1, 1∶200, Abcam, ab2725).
Expressing:Article Title: Generating GABAergic neurons in brains
Article Snippet: Antibodies The following primary antibodies were used: polyclonal anti-green fluorescent protein (GFP, chicken, 1:2000, Abcam, AB13970), polyclonal anti-glial fibrillary acidic protein (GFAP, rabbit, 1:1000, Dako, Z0334), monoclonal anti-CNPase (mouse, 1:800, Abcam, AB6319), polyclonal anti-vesicular glutamate transporter 1 (vGluT1, rabbit, 1:1000, Synaptic Systems), monoclonal anti GAD67 (mouse, 1:1000, Millipore, MAB5406), monoclonal anti GAD65 (GAD-6, mouse, 1:1000, Developmental Studies Hybridoma Bank, Iowa City), monoclonal anti-NG2 (mouse, 1:600, Abcam, AB50009), polyclonal anti-Iba1 (rabbit, 1:1000, Wako, 019-19741), polyclonal anti-NeuN (rabbit, 1:1000, Millipore, ABN78), monoclonal anti-βIII tubulin (Tuj1, mouse, 1:1000, COVANCE, MMS-435P), polyclonal anti-γ-aminobutyric acid (GABA, rabbit, 1:2000, Sigma, A2052), polyclonal anti-Red Fluorescent Protein (RFP, rat, 1:1500, antibody-online, ABIN334653; and rabbit, 1:1000, Rockland, 600-401-379), polyclonal anti-T-box brain 1 (Tbr1, 1:800, rabbit, Abcam, AB31940), monoclonal anti-Ctip2 (Rat, 1:1000, Abcam, AB18465), monoclonal anti-NeuroD1 (mouse, 1:1000, Abcam, AB60704), polyclonal anti-Dlx2 (rabbit, 1:600, Abcam, AB30339), polyclonal anti-Doublecortin (DCX, rabbit, 1:1000, Abcam, AB18723), monoclonal anti-Parvalbumin (PV, mouse, 1:1000, sigma, P3088), polyclonal anti-Calretinin (CR, goat, 1:1500, Millipore, AB1550), monoclonal anti-Somatostatin (SST, rat, 1:300, Millipore, MAB354), polyclonal anti-Cholecystokinin (Catalog Nos.
Article Title: Directing Astroglia from the Cerebral Cortex into Subtype Specific Functional Neurons
Article Snippet: The following primary antibodies were used: anti-GFP (GFP, chicken, 1∶1000, Aves Labs, GFP-1020 ), polyclonal anti-Glial Fibrillary Acidic Protein (GFAP, rabbit, 1∶4000, DakoCytomation, Z0334 ), polyclonal anti-Red Fluorescent Protein (RFP, rabbit, 1∶500, Chemicon, AB3216 ), polyclonal anti-RFP (rabbit, Rockland, 1∶2000, 600-401-379 ), polyclonal anti-vesicular glutamate transporter 1 (vGluT11, rabbit, 1∶1000, Synaptic Systems, 135302 ), monoclonal anti-Microtubule Associated Protein 2 (MAP2, mouse IgG1, 1∶200, Sigma-Aldrich, M4403 ), monoclonal anti-synapsin 1 (mouse IgG2, 1∶2000, Synaptic Systems, 106001 ), polyclonal anti-vGaT (guinea pig, 1∶200, Synaptic Systems, 131004 ), polyclonal anti-Tbr1 (rabbit, 1∶1000, Millipore, AB9616), polyclonal anti-Tbr2 (rabbit, 1∶500, Millipore, AB9618), monoclonal anti-βIII tubulin (mouse IgG2b, 1∶500, Sigma, T8660), polyclonal anti-GAD1(GAD67) (rabbit, 1∶500, Synaptic Systems, 198013), monoclonal anti-calretinin (mouse IgG1, 1∶200, Millipore, MAB1568), and monoclonal anti-CaM kinase IIα (mouse IgG1, 1∶200, Abcam, ab2725).
Injection:Article Title: Generating GABAergic neurons in brains
Article Snippet: Antibodies The following primary antibodies were used: polyclonal anti-green fluorescent protein (GFP, chicken, 1:2000, Abcam, AB13970), polyclonal anti-glial fibrillary acidic protein (GFAP, rabbit, 1:1000, Dako, Z0334), monoclonal anti-CNPase (mouse, 1:800, Abcam, AB6319), polyclonal anti-vesicular glutamate transporter 1 (vGluT1, rabbit, 1:1000, Synaptic Systems), monoclonal anti GAD67 (mouse, 1:1000, Millipore, MAB5406), monoclonal anti GAD65 (GAD-6, mouse, 1:1000, Developmental Studies Hybridoma Bank, Iowa City), monoclonal anti-NG2 (mouse, 1:600, Abcam, AB50009), polyclonal anti-Iba1 (rabbit, 1:1000, Wako, 019-19741), polyclonal anti-NeuN (rabbit, 1:1000, Millipore, ABN78), monoclonal anti-βIII tubulin (Tuj1, mouse, 1:1000, COVANCE, MMS-435P), polyclonal anti-γ-aminobutyric acid (GABA, rabbit, 1:2000, Sigma, A2052), polyclonal anti-Red Fluorescent Protein (RFP, rat, 1:1500, antibody-online, ABIN334653; and rabbit, 1:1000, Rockland, 600-401-379), polyclonal anti-T-box brain 1 (Tbr1, 1:800, rabbit, Abcam, AB31940), monoclonal anti-Ctip2 (Rat, 1:1000, Abcam, AB18465), monoclonal anti-NeuroD1 (mouse, 1:1000, Abcam, AB60704), polyclonal anti-Dlx2 (rabbit, 1:600, Abcam, AB30339), polyclonal anti-Doublecortin (DCX, rabbit, 1:1000, Abcam, AB18723), monoclonal anti-Parvalbumin (PV, mouse, 1:1000, sigma, P3088), polyclonal anti-Calretinin (CR, goat, 1:1500, Millipore, AB1550), monoclonal anti-Somatostatin (SST, rat, 1:300, Millipore, MAB354), polyclonal anti-Cholecystokinin (Catalog Nos.
Article Title: Directing Astroglia from the Cerebral Cortex into Subtype Specific Functional Neurons
Article Snippet: The following primary antibodies were used: anti-GFP (GFP, chicken, 1∶1000, Aves Labs, GFP-1020 ), polyclonal anti-Glial Fibrillary Acidic Protein (GFAP, rabbit, 1∶4000, DakoCytomation, Z0334 ), polyclonal anti-Red Fluorescent Protein (RFP, rabbit, 1∶500, Chemicon, AB3216 ), polyclonal anti-RFP (rabbit, Rockland, 1∶2000, 600-401-379 ), polyclonal anti-vesicular glutamate transporter 1 (vGluT11, rabbit, 1∶1000, Synaptic Systems, 135302 ), monoclonal anti-Microtubule Associated Protein 2 (MAP2, mouse IgG1, 1∶200, Sigma-Aldrich, M4403 ), monoclonal anti-synapsin 1 (mouse IgG2, 1∶2000, Synaptic Systems, 106001 ), polyclonal anti-vGaT (guinea pig, 1∶200, Synaptic Systems, 131004 ), polyclonal anti-Tbr1 (rabbit, 1∶1000, Millipore, AB9616), polyclonal anti-Tbr2 (rabbit, 1∶500, Millipore, AB9618), monoclonal anti-βIII tubulin (mouse IgG2b, 1∶500, Sigma, T8660), polyclonal anti-GAD1(GAD67) (rabbit, 1∶500, Synaptic Systems, 198013), monoclonal anti-calretinin (mouse IgG1, 1∶200, Millipore, MAB1568), and monoclonal anti-CaM kinase IIα (mouse IgG1, 1∶200, Abcam, ab2725).
Transmission Assay:Article Title: Generating GABAergic neurons in brains
Article Snippet: Antibodies The following primary antibodies were used: polyclonal anti-green fluorescent protein (GFP, chicken, 1:2000, Abcam, AB13970), polyclonal anti-glial fibrillary acidic protein (GFAP, rabbit, 1:1000, Dako, Z0334), monoclonal anti-CNPase (mouse, 1:800, Abcam, AB6319), polyclonal anti-vesicular glutamate transporter 1 (vGluT1, rabbit, 1:1000, Synaptic Systems), monoclonal anti GAD67 (mouse, 1:1000, Millipore, MAB5406), monoclonal anti GAD65 (GAD-6, mouse, 1:1000, Developmental Studies Hybridoma Bank, Iowa City), monoclonal anti-NG2 (mouse, 1:600, Abcam, AB50009), polyclonal anti-Iba1 (rabbit, 1:1000, Wako, 019-19741), polyclonal anti-NeuN (rabbit, 1:1000, Millipore, ABN78), monoclonal anti-βIII tubulin (Tuj1, mouse, 1:1000, COVANCE, MMS-435P), polyclonal anti-γ-aminobutyric acid (GABA, rabbit, 1:2000, Sigma, A2052), polyclonal anti-Red Fluorescent Protein (RFP, rat, 1:1500, antibody-online, ABIN334653; and rabbit, 1:1000, Rockland, 600-401-379), polyclonal anti-T-box brain 1 (Tbr1, 1:800, rabbit, Abcam, AB31940), monoclonal anti-Ctip2 (Rat, 1:1000, Abcam, AB18465), monoclonal anti-NeuroD1 (mouse, 1:1000, Abcam, AB60704), polyclonal anti-Dlx2 (rabbit, 1:600, Abcam, AB30339), polyclonal anti-Doublecortin (DCX, rabbit, 1:1000, Abcam, AB18723), monoclonal anti-Parvalbumin (PV, mouse, 1:1000, sigma, P3088), polyclonal anti-Calretinin (CR, goat, 1:1500, Millipore, AB1550), monoclonal anti-Somatostatin (SST, rat, 1:300, Millipore, MAB354), polyclonal anti-Cholecystokinin (Catalog Nos.
Article Title: Directing Astroglia from the Cerebral Cortex into Subtype Specific Functional Neurons
Article Snippet: The following primary antibodies were used: anti-GFP (GFP, chicken, 1∶1000, Aves Labs, GFP-1020 ), polyclonal anti-Glial Fibrillary Acidic Protein (GFAP, rabbit, 1∶4000, DakoCytomation, Z0334 ), polyclonal anti-Red Fluorescent Protein (RFP, rabbit, 1∶500, Chemicon, AB3216 ), polyclonal anti-RFP (rabbit, Rockland, 1∶2000, 600-401-379 ), polyclonal anti-vesicular glutamate transporter 1 (vGluT11, rabbit, 1∶1000, Synaptic Systems, 135302 ), monoclonal anti-Microtubule Associated Protein 2 (MAP2, mouse IgG1, 1∶200, Sigma-Aldrich, M4403 ), monoclonal anti-synapsin 1 (mouse IgG2, 1∶2000, Synaptic Systems, 106001 ), polyclonal anti-vGaT (guinea pig, 1∶200, Synaptic Systems, 131004 ), polyclonal anti-Tbr1 (rabbit, 1∶1000, Millipore, AB9616), polyclonal anti-Tbr2 (rabbit, 1∶500, Millipore, AB9618), monoclonal anti-βIII tubulin (mouse IgG2b, 1∶500, Sigma, T8660), polyclonal anti-GAD1(GAD67) (rabbit, 1∶500, Synaptic Systems, 198013), monoclonal anti-calretinin (mouse IgG1, 1∶200, Millipore, MAB1568), and monoclonal anti-CaM kinase IIα (mouse IgG1, 1∶200, Abcam, ab2725).
Cell Culture:Article Title: Generating GABAergic neurons in brains
Article Snippet: Antibodies The following primary antibodies were used: polyclonal anti-green fluorescent protein (GFP, chicken, 1:2000, Abcam, AB13970), polyclonal anti-glial fibrillary acidic protein (GFAP, rabbit, 1:1000, Dako, Z0334), monoclonal anti-CNPase (mouse, 1:800, Abcam, AB6319), polyclonal anti-vesicular glutamate transporter 1 (vGluT1, rabbit, 1:1000, Synaptic Systems), monoclonal anti GAD67 (mouse, 1:1000, Millipore, MAB5406), monoclonal anti GAD65 (GAD-6, mouse, 1:1000, Developmental Studies Hybridoma Bank, Iowa City), monoclonal anti-NG2 (mouse, 1:600, Abcam, AB50009), polyclonal anti-Iba1 (rabbit, 1:1000, Wako, 019-19741), polyclonal anti-NeuN (rabbit, 1:1000, Millipore, ABN78), monoclonal anti-βIII tubulin (Tuj1, mouse, 1:1000, COVANCE, MMS-435P), polyclonal anti-γ-aminobutyric acid (GABA, rabbit, 1:2000, Sigma, A2052), polyclonal anti-Red Fluorescent Protein (RFP, rat, 1:1500, antibody-online, ABIN334653; and rabbit, 1:1000, Rockland, 600-401-379), polyclonal anti-T-box brain 1 (Tbr1, 1:800, rabbit, Abcam, AB31940), monoclonal anti-Ctip2 (Rat, 1:1000, Abcam, AB18465), monoclonal anti-NeuroD1 (mouse, 1:1000, Abcam, AB60704), polyclonal anti-Dlx2 (rabbit, 1:600, Abcam, AB30339), polyclonal anti-Doublecortin (DCX, rabbit, 1:1000, Abcam, AB18723), monoclonal anti-Parvalbumin (PV, mouse, 1:1000, sigma, P3088), polyclonal anti-Calretinin (CR, goat, 1:1500, Millipore, AB1550), monoclonal anti-Somatostatin (SST, rat, 1:300, Millipore, MAB354), polyclonal anti-Cholecystokinin (Catalog Nos.
Article Title: Directing Astroglia from the Cerebral Cortex into Subtype Specific Functional Neurons
Article Snippet: The following primary antibodies were used: anti-GFP (GFP, chicken, 1∶1000, Aves Labs, GFP-1020 ), polyclonal anti-Glial Fibrillary Acidic Protein (GFAP, rabbit, 1∶4000, DakoCytomation, Z0334 ), polyclonal anti-Red Fluorescent Protein (RFP, rabbit, 1∶500, Chemicon, AB3216 ), polyclonal anti-RFP (rabbit, Rockland, 1∶2000, 600-401-379 ), polyclonal anti-vesicular glutamate transporter 1 (vGluT11, rabbit, 1∶1000, Synaptic Systems, 135302 ), monoclonal anti-Microtubule Associated Protein 2 (MAP2, mouse IgG1, 1∶200, Sigma-Aldrich, M4403 ), monoclonal anti-synapsin 1 (mouse IgG2, 1∶2000, Synaptic Systems, 106001 ), polyclonal anti-vGaT (guinea pig, 1∶200, Synaptic Systems, 131004 ), polyclonal anti-Tbr1 (rabbit, 1∶1000, Millipore, AB9616), polyclonal anti-Tbr2 (rabbit, 1∶500, Millipore, AB9618), monoclonal anti-βIII tubulin (mouse IgG2b, 1∶500, Sigma, T8660), polyclonal anti-GAD1(GAD67) (rabbit, 1∶500, Synaptic Systems, 198013), monoclonal anti-calretinin (mouse IgG1, 1∶200, Millipore, MAB1568), and monoclonal anti-CaM kinase IIα (mouse IgG1, 1∶200, Abcam, ab2725).
Transferring:Article Title: Generating GABAergic neurons in brains
Article Snippet: Antibodies The following primary antibodies were used: polyclonal anti-green fluorescent protein (GFP, chicken, 1:2000, Abcam, AB13970), polyclonal anti-glial fibrillary acidic protein (GFAP, rabbit, 1:1000, Dako, Z0334), monoclonal anti-CNPase (mouse, 1:800, Abcam, AB6319), polyclonal anti-vesicular glutamate transporter 1 (vGluT1, rabbit, 1:1000, Synaptic Systems), monoclonal anti GAD67 (mouse, 1:1000, Millipore, MAB5406), monoclonal anti GAD65 (GAD-6, mouse, 1:1000, Developmental Studies Hybridoma Bank, Iowa City), monoclonal anti-NG2 (mouse, 1:600, Abcam, AB50009), polyclonal anti-Iba1 (rabbit, 1:1000, Wako, 019-19741), polyclonal anti-NeuN (rabbit, 1:1000, Millipore, ABN78), monoclonal anti-βIII tubulin (Tuj1, mouse, 1:1000, COVANCE, MMS-435P), polyclonal anti-γ-aminobutyric acid (GABA, rabbit, 1:2000, Sigma, A2052), polyclonal anti-Red Fluorescent Protein (RFP, rat, 1:1500, antibody-online, ABIN334653; and rabbit, 1:1000, Rockland, 600-401-379), polyclonal anti-T-box brain 1 (Tbr1, 1:800, rabbit, Abcam, AB31940), monoclonal anti-Ctip2 (Rat, 1:1000, Abcam, AB18465), monoclonal anti-NeuroD1 (mouse, 1:1000, Abcam, AB60704), polyclonal anti-Dlx2 (rabbit, 1:600, Abcam, AB30339), polyclonal anti-Doublecortin (DCX, rabbit, 1:1000, Abcam, AB18723), monoclonal anti-Parvalbumin (PV, mouse, 1:1000, sigma, P3088), polyclonal anti-Calretinin (CR, goat, 1:1500, Millipore, AB1550), monoclonal anti-Somatostatin (SST, rat, 1:300, Millipore, MAB354), polyclonal anti-Cholecystokinin (Catalog Nos.
Article Title: Directing Astroglia from the Cerebral Cortex into Subtype Specific Functional Neurons
Article Snippet: The following primary antibodies were used: anti-GFP (GFP, chicken, 1∶1000, Aves Labs, GFP-1020 ), polyclonal anti-Glial Fibrillary Acidic Protein (GFAP, rabbit, 1∶4000, DakoCytomation, Z0334 ), polyclonal anti-Red Fluorescent Protein (RFP, rabbit, 1∶500, Chemicon, AB3216 ), polyclonal anti-RFP (rabbit, Rockland, 1∶2000, 600-401-379 ), polyclonal anti-vesicular glutamate transporter 1 (vGluT11, rabbit, 1∶1000, Synaptic Systems, 135302 ), monoclonal anti-Microtubule Associated Protein 2 (MAP2, mouse IgG1, 1∶200, Sigma-Aldrich, M4403 ), monoclonal anti-synapsin 1 (mouse IgG2, 1∶2000, Synaptic Systems, 106001 ), polyclonal anti-vGaT (guinea pig, 1∶200, Synaptic Systems, 131004 ), polyclonal anti-Tbr1 (rabbit, 1∶1000, Millipore, AB9616), polyclonal anti-Tbr2 (rabbit, 1∶500, Millipore, AB9618), monoclonal anti-βIII tubulin (mouse IgG2b, 1∶500, Sigma, T8660), polyclonal anti-GAD1(GAD67) (rabbit, 1∶500, Synaptic Systems, 198013), monoclonal anti-calretinin (mouse IgG1, 1∶200, Millipore, MAB1568), and monoclonal anti-CaM kinase IIα (mouse IgG1, 1∶200, Abcam, ab2725).
Sequencing:Article Title: Generating GABAergic neurons in brains
Article Snippet: Antibodies The following primary antibodies were used: polyclonal anti-green fluorescent protein (GFP, chicken, 1:2000, Abcam, AB13970), polyclonal anti-glial fibrillary acidic protein (GFAP, rabbit, 1:1000, Dako, Z0334), monoclonal anti-CNPase (mouse, 1:800, Abcam, AB6319), polyclonal anti-vesicular glutamate transporter 1 (vGluT1, rabbit, 1:1000, Synaptic Systems), monoclonal anti GAD67 (mouse, 1:1000, Millipore, MAB5406), monoclonal anti GAD65 (GAD-6, mouse, 1:1000, Developmental Studies Hybridoma Bank, Iowa City), monoclonal anti-NG2 (mouse, 1:600, Abcam, AB50009), polyclonal anti-Iba1 (rabbit, 1:1000, Wako, 019-19741), polyclonal anti-NeuN (rabbit, 1:1000, Millipore, ABN78), monoclonal anti-βIII tubulin (Tuj1, mouse, 1:1000, COVANCE, MMS-435P), polyclonal anti-γ-aminobutyric acid (GABA, rabbit, 1:2000, Sigma, A2052), polyclonal anti-Red Fluorescent Protein (RFP, rat, 1:1500, antibody-online, ABIN334653; and rabbit, 1:1000, Rockland, 600-401-379), polyclonal anti-T-box brain 1 (Tbr1, 1:800, rabbit, Abcam, AB31940), monoclonal anti-Ctip2 (Rat, 1:1000, Abcam, AB18465), monoclonal anti-NeuroD1 (mouse, 1:1000, Abcam, AB60704), polyclonal anti-Dlx2 (rabbit, 1:600, Abcam, AB30339), polyclonal anti-Doublecortin (DCX, rabbit, 1:1000, Abcam, AB18723), monoclonal anti-Parvalbumin (PV, mouse, 1:1000, sigma, P3088), polyclonal anti-Calretinin (CR, goat, 1:1500, Millipore, AB1550), monoclonal anti-Somatostatin (SST, rat, 1:300, Millipore, MAB354), polyclonal anti-Cholecystokinin (Catalog Nos.
Article Title: Directing Astroglia from the Cerebral Cortex into Subtype Specific Functional Neurons
Article Snippet: The following primary antibodies were used: anti-GFP (GFP, chicken, 1∶1000, Aves Labs, GFP-1020 ), polyclonal anti-Glial Fibrillary Acidic Protein (GFAP, rabbit, 1∶4000, DakoCytomation, Z0334 ), polyclonal anti-Red Fluorescent Protein (RFP, rabbit, 1∶500, Chemicon, AB3216 ), polyclonal anti-RFP (rabbit, Rockland, 1∶2000, 600-401-379 ), polyclonal anti-vesicular glutamate transporter 1 (vGluT11, rabbit, 1∶1000, Synaptic Systems, 135302 ), monoclonal anti-Microtubule Associated Protein 2 (MAP2, mouse IgG1, 1∶200, Sigma-Aldrich, M4403 ), monoclonal anti-synapsin 1 (mouse IgG2, 1∶2000, Synaptic Systems, 106001 ), polyclonal anti-vGaT (guinea pig, 1∶200, Synaptic Systems, 131004 ), polyclonal anti-Tbr1 (rabbit, 1∶1000, Millipore, AB9616), polyclonal anti-Tbr2 (rabbit, 1∶500, Millipore, AB9618), monoclonal anti-βIII tubulin (mouse IgG2b, 1∶500, Sigma, T8660), polyclonal anti-GAD1(GAD67) (rabbit, 1∶500, Synaptic Systems, 198013), monoclonal anti-calretinin (mouse IgG1, 1∶200, Millipore, MAB1568), and monoclonal anti-CaM kinase IIα (mouse IgG1, 1∶200, Abcam, ab2725).
Isolation:Article Title: Generating GABAergic neurons in brains
Article Snippet: Antibodies The following primary antibodies were used: polyclonal anti-green fluorescent protein (GFP, chicken, 1:2000, Abcam, AB13970), polyclonal anti-glial fibrillary acidic protein (GFAP, rabbit, 1:1000, Dako, Z0334), monoclonal anti-CNPase (mouse, 1:800, Abcam, AB6319), polyclonal anti-vesicular glutamate transporter 1 (vGluT1, rabbit, 1:1000, Synaptic Systems), monoclonal anti GAD67 (mouse, 1:1000, Millipore, MAB5406), monoclonal anti GAD65 (GAD-6, mouse, 1:1000, Developmental Studies Hybridoma Bank, Iowa City), monoclonal anti-NG2 (mouse, 1:600, Abcam, AB50009), polyclonal anti-Iba1 (rabbit, 1:1000, Wako, 019-19741), polyclonal anti-NeuN (rabbit, 1:1000, Millipore, ABN78), monoclonal anti-βIII tubulin (Tuj1, mouse, 1:1000, COVANCE, MMS-435P), polyclonal anti-γ-aminobutyric acid (GABA, rabbit, 1:2000, Sigma, A2052), polyclonal anti-Red Fluorescent Protein (RFP, rat, 1:1500, antibody-online, ABIN334653; and rabbit, 1:1000, Rockland, 600-401-379), polyclonal anti-T-box brain 1 (Tbr1, 1:800, rabbit, Abcam, AB31940), monoclonal anti-Ctip2 (Rat, 1:1000, Abcam, AB18465), monoclonal anti-NeuroD1 (mouse, 1:1000, Abcam, AB60704), polyclonal anti-Dlx2 (rabbit, 1:600, Abcam, AB30339), polyclonal anti-Doublecortin (DCX, rabbit, 1:1000, Abcam, AB18723), monoclonal anti-Parvalbumin (PV, mouse, 1:1000, sigma, P3088), polyclonal anti-Calretinin (CR, goat, 1:1500, Millipore, AB1550), monoclonal anti-Somatostatin (SST, rat, 1:300, Millipore, MAB354), polyclonal anti-Cholecystokinin (Catalog Nos.
Article Title: Directing Astroglia from the Cerebral Cortex into Subtype Specific Functional Neurons
Article Snippet: The following primary antibodies were used: anti-GFP (GFP, chicken, 1∶1000, Aves Labs, GFP-1020 ), polyclonal anti-Glial Fibrillary Acidic Protein (GFAP, rabbit, 1∶4000, DakoCytomation, Z0334 ), polyclonal anti-Red Fluorescent Protein (RFP, rabbit, 1∶500, Chemicon, AB3216 ), polyclonal anti-RFP (rabbit, Rockland, 1∶2000, 600-401-379 ), polyclonal anti-vesicular glutamate transporter 1 (vGluT11, rabbit, 1∶1000, Synaptic Systems, 135302 ), monoclonal anti-Microtubule Associated Protein 2 (MAP2, mouse IgG1, 1∶200, Sigma-Aldrich, M4403 ), monoclonal anti-synapsin 1 (mouse IgG2, 1∶2000, Synaptic Systems, 106001 ), polyclonal anti-vGaT (guinea pig, 1∶200, Synaptic Systems, 131004 ), polyclonal anti-Tbr1 (rabbit, 1∶1000, Millipore, AB9616), polyclonal anti-Tbr2 (rabbit, 1∶500, Millipore, AB9618), monoclonal anti-βIII tubulin (mouse IgG2b, 1∶500, Sigma, T8660), polyclonal anti-GAD1(GAD67) (rabbit, 1∶500, Synaptic Systems, 198013), monoclonal anti-calretinin (mouse IgG1, 1∶200, Millipore, MAB1568), and monoclonal anti-CaM kinase IIα (mouse IgG1, 1∶200, Abcam, ab2725).
Immunocytochemistry:Article Title: Generating GABAergic neurons in brains
Article Snippet: Antibodies The following primary antibodies were used: polyclonal anti-green fluorescent protein (GFP, chicken, 1:2000, Abcam, AB13970), polyclonal anti-glial fibrillary acidic protein (GFAP, rabbit, 1:1000, Dako, Z0334), monoclonal anti-CNPase (mouse, 1:800, Abcam, AB6319), polyclonal anti-vesicular glutamate transporter 1 (vGluT1, rabbit, 1:1000, Synaptic Systems), monoclonal anti GAD67 (mouse, 1:1000, Millipore, MAB5406), monoclonal anti GAD65 (GAD-6, mouse, 1:1000, Developmental Studies Hybridoma Bank, Iowa City), monoclonal anti-NG2 (mouse, 1:600, Abcam, AB50009), polyclonal anti-Iba1 (rabbit, 1:1000, Wako, 019-19741), polyclonal anti-NeuN (rabbit, 1:1000, Millipore, ABN78), monoclonal anti-βIII tubulin (Tuj1, mouse, 1:1000, COVANCE, MMS-435P), polyclonal anti-γ-aminobutyric acid (GABA, rabbit, 1:2000, Sigma, A2052), polyclonal anti-Red Fluorescent Protein (RFP, rat, 1:1500, antibody-online, ABIN334653; and rabbit, 1:1000, Rockland, 600-401-379), polyclonal anti-T-box brain 1 (Tbr1, 1:800, rabbit, Abcam, AB31940), monoclonal anti-Ctip2 (Rat, 1:1000, Abcam, AB18465), monoclonal anti-NeuroD1 (mouse, 1:1000, Abcam, AB60704), polyclonal anti-Dlx2 (rabbit, 1:600, Abcam, AB30339), polyclonal anti-Doublecortin (DCX, rabbit, 1:1000, Abcam, AB18723), monoclonal anti-Parvalbumin (PV, mouse, 1:1000, sigma, P3088), polyclonal anti-Calretinin (CR, goat, 1:1500, Millipore, AB1550), monoclonal anti-Somatostatin (SST, rat, 1:300, Millipore, MAB354), polyclonal anti-Cholecystokinin (Catalog Nos.
Article Title: Directing Astroglia from the Cerebral Cortex into Subtype Specific Functional Neurons
Article Snippet: The following primary antibodies were used: anti-GFP (GFP, chicken, 1∶1000, Aves Labs, GFP-1020 ), polyclonal anti-Glial Fibrillary Acidic Protein (GFAP, rabbit, 1∶4000, DakoCytomation, Z0334 ), polyclonal anti-Red Fluorescent Protein (RFP, rabbit, 1∶500, Chemicon, AB3216 ), polyclonal anti-RFP (rabbit, Rockland, 1∶2000, 600-401-379 ), polyclonal anti-vesicular glutamate transporter 1 (vGluT11, rabbit, 1∶1000, Synaptic Systems, 135302 ), monoclonal anti-Microtubule Associated Protein 2 (MAP2, mouse IgG1, 1∶200, Sigma-Aldrich, M4403 ), monoclonal anti-synapsin 1 (mouse IgG2, 1∶2000, Synaptic Systems, 106001 ), polyclonal anti-vGaT (guinea pig, 1∶200, Synaptic Systems, 131004 ), polyclonal anti-Tbr1 (rabbit, 1∶1000, Millipore, AB9616), polyclonal anti-Tbr2 (rabbit, 1∶500, Millipore, AB9618), monoclonal anti-βIII tubulin (mouse IgG2b, 1∶500, Sigma, T8660), polyclonal anti-GAD1(GAD67) (rabbit, 1∶500, Synaptic Systems, 198013), monoclonal anti-calretinin (mouse IgG1, 1∶200, Millipore, MAB1568), and monoclonal anti-CaM kinase IIα (mouse IgG1, 1∶200, Abcam, ab2725).
Staining:Article Title: Generating GABAergic neurons in brains
Article Snippet: Antibodies The following primary antibodies were used: polyclonal anti-green fluorescent protein (GFP, chicken, 1:2000, Abcam, AB13970), polyclonal anti-glial fibrillary acidic protein (GFAP, rabbit, 1:1000, Dako, Z0334), monoclonal anti-CNPase (mouse, 1:800, Abcam, AB6319), polyclonal anti-vesicular glutamate transporter 1 (vGluT1, rabbit, 1:1000, Synaptic Systems), monoclonal anti GAD67 (mouse, 1:1000, Millipore, MAB5406), monoclonal anti GAD65 (GAD-6, mouse, 1:1000, Developmental Studies Hybridoma Bank, Iowa City), monoclonal anti-NG2 (mouse, 1:600, Abcam, AB50009), polyclonal anti-Iba1 (rabbit, 1:1000, Wako, 019-19741), polyclonal anti-NeuN (rabbit, 1:1000, Millipore, ABN78), monoclonal anti-βIII tubulin (Tuj1, mouse, 1:1000, COVANCE, MMS-435P), polyclonal anti-γ-aminobutyric acid (GABA, rabbit, 1:2000, Sigma, A2052), polyclonal anti-Red Fluorescent Protein (RFP, rat, 1:1500, antibody-online, ABIN334653; and rabbit, 1:1000, Rockland, 600-401-379), polyclonal anti-T-box brain 1 (Tbr1, 1:800, rabbit, Abcam, AB31940), monoclonal anti-Ctip2 (Rat, 1:1000, Abcam, AB18465), monoclonal anti-NeuroD1 (mouse, 1:1000, Abcam, AB60704), polyclonal anti-Dlx2 (rabbit, 1:600, Abcam, AB30339), polyclonal anti-Doublecortin (DCX, rabbit, 1:1000, Abcam, AB18723), monoclonal anti-Parvalbumin (PV, mouse, 1:1000, sigma, P3088), polyclonal anti-Calretinin (CR, goat, 1:1500, Millipore, AB1550), monoclonal anti-Somatostatin (SST, rat, 1:300, Millipore, MAB354), polyclonal anti-Cholecystokinin (Catalog Nos.
Article Title: Directing Astroglia from the Cerebral Cortex into Subtype Specific Functional Neurons
Article Snippet: The following primary antibodies were used: anti-GFP (GFP, chicken, 1∶1000, Aves Labs, GFP-1020 ), polyclonal anti-Glial Fibrillary Acidic Protein (GFAP, rabbit, 1∶4000, DakoCytomation, Z0334 ), polyclonal anti-Red Fluorescent Protein (RFP, rabbit, 1∶500, Chemicon, AB3216 ), polyclonal anti-RFP (rabbit, Rockland, 1∶2000, 600-401-379 ), polyclonal anti-vesicular glutamate transporter 1 (vGluT11, rabbit, 1∶1000, Synaptic Systems, 135302 ), monoclonal anti-Microtubule Associated Protein 2 (MAP2, mouse IgG1, 1∶200, Sigma-Aldrich, M4403 ), monoclonal anti-synapsin 1 (mouse IgG2, 1∶2000, Synaptic Systems, 106001 ), polyclonal anti-vGaT (guinea pig, 1∶200, Synaptic Systems, 131004 ), polyclonal anti-Tbr1 (rabbit, 1∶1000, Millipore, AB9616), polyclonal anti-Tbr2 (rabbit, 1∶500, Millipore, AB9618), monoclonal anti-βIII tubulin (mouse IgG2b, 1∶500, Sigma, T8660), polyclonal anti-GAD1(GAD67) (rabbit, 1∶500, Synaptic Systems, 198013), monoclonal anti-calretinin (mouse IgG1, 1∶200, Millipore, MAB1568), and monoclonal anti-CaM kinase IIα (mouse IgG1, 1∶200, Abcam, ab2725).
Quantitative RT-PCR:Article Title: Generating GABAergic neurons in brains
Article Snippet: Antibodies The following primary antibodies were used: polyclonal anti-green fluorescent protein (GFP, chicken, 1:2000, Abcam, AB13970), polyclonal anti-glial fibrillary acidic protein (GFAP, rabbit, 1:1000, Dako, Z0334), monoclonal anti-CNPase (mouse, 1:800, Abcam, AB6319), polyclonal anti-vesicular glutamate transporter 1 (vGluT1, rabbit, 1:1000, Synaptic Systems), monoclonal anti GAD67 (mouse, 1:1000, Millipore, MAB5406), monoclonal anti GAD65 (GAD-6, mouse, 1:1000, Developmental Studies Hybridoma Bank, Iowa City), monoclonal anti-NG2 (mouse, 1:600, Abcam, AB50009), polyclonal anti-Iba1 (rabbit, 1:1000, Wako, 019-19741), polyclonal anti-NeuN (rabbit, 1:1000, Millipore, ABN78), monoclonal anti-βIII tubulin (Tuj1, mouse, 1:1000, COVANCE, MMS-435P), polyclonal anti-γ-aminobutyric acid (GABA, rabbit, 1:2000, Sigma, A2052), polyclonal anti-Red Fluorescent Protein (RFP, rat, 1:1500, antibody-online, ABIN334653; and rabbit, 1:1000, Rockland, 600-401-379), polyclonal anti-T-box brain 1 (Tbr1, 1:800, rabbit, Abcam, AB31940), monoclonal anti-Ctip2 (Rat, 1:1000, Abcam, AB18465), monoclonal anti-NeuroD1 (mouse, 1:1000, Abcam, AB60704), polyclonal anti-Dlx2 (rabbit, 1:600, Abcam, AB30339), polyclonal anti-Doublecortin (DCX, rabbit, 1:1000, Abcam, AB18723), monoclonal anti-Parvalbumin (PV, mouse, 1:1000, sigma, P3088), polyclonal anti-Calretinin (CR, goat, 1:1500, Millipore, AB1550), monoclonal anti-Somatostatin (SST, rat, 1:300, Millipore, MAB354), polyclonal anti-Cholecystokinin (Catalog Nos.
Article Title: Directing Astroglia from the Cerebral Cortex into Subtype Specific Functional Neurons
Article Snippet: The following primary antibodies were used: anti-GFP (GFP, chicken, 1∶1000, Aves Labs, GFP-1020 ), polyclonal anti-Glial Fibrillary Acidic Protein (GFAP, rabbit, 1∶4000, DakoCytomation, Z0334 ), polyclonal anti-Red Fluorescent Protein (RFP, rabbit, 1∶500, Chemicon, AB3216 ), polyclonal anti-RFP (rabbit, Rockland, 1∶2000, 600-401-379 ), polyclonal anti-vesicular glutamate transporter 1 (vGluT11, rabbit, 1∶1000, Synaptic Systems, 135302 ), monoclonal anti-Microtubule Associated Protein 2 (MAP2, mouse IgG1, 1∶200, Sigma-Aldrich, M4403 ), monoclonal anti-synapsin 1 (mouse IgG2, 1∶2000, Synaptic Systems, 106001 ), polyclonal anti-vGaT (guinea pig, 1∶200, Synaptic Systems, 131004 ), polyclonal anti-Tbr1 (rabbit, 1∶1000, Millipore, AB9616), polyclonal anti-Tbr2 (rabbit, 1∶500, Millipore, AB9618), monoclonal anti-βIII tubulin (mouse IgG2b, 1∶500, Sigma, T8660), polyclonal anti-GAD1(GAD67) (rabbit, 1∶500, Synaptic Systems, 198013), monoclonal anti-calretinin (mouse IgG1, 1∶200, Millipore, MAB1568), and monoclonal anti-CaM kinase IIα (mouse IgG1, 1∶200, Abcam, ab2725).
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